Paraphyletic genus Ditylenchus Filipjev (Nematoda, Tylenchida), corresponding to the D. triformis-group and the D. dipsaci-group scheme
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چکیده
The genus Ditylenchus has been divided into 2 groups: the Ditylenchus triformis-group, and the Ditylenchus dipsaci-group based on morphological and biological characters. A total of 18 populations belong to 5 species of Ditylenchus was studied: Ditylenchus africanus, Ditylenchus destructor, Ditylenchus myceliophagus and dipsaci, Ditylenchus weischeri, the first 3 belong to the Ditylenchus triformis-group, the last 2 the Ditylenchus dipsaci-group. The species of Ditylenchus triformis-group were cultured on fungi, while the species from Ditylenchus dispaci-group cultured on excised roots of plant hosts in petri dish. DNA sequences of regions of the nuclear ribosomal first internal transcribed spacer (ITS1) and the small subunit 18S were PCR amplified, sequenced and the phylogenetic analyses also including the sequences of the closely related species from the GenBank. The randomly amplified polymorphisms of genomic DNA (RAPD) were also generated. Two clusters or clades corresponding to the 2 groups were consistently observed with significant statistical support from the 3 datasets. The phylogenetic analysis also revealed that the genus is paraphyletic, separating the 2 groups by species of Anguina and Subanguina.
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Management of Ditylenchus dipsaci in Daffodils with Foliar Applications of Oxamyl.
Ditylenchus dipsaci (Kühn) Filipjev is a serious pest of commercial daffodil (Narcissus sp.) in northern California. The control measures practiced by growers in recent years are postharvest treatment of planting bulbs for 3 hours in a 1% solution of formalin at 44 C combined with preplant soil fumigation with 1,3-dicbloropropene and (or) at-planting applications of phorate. In field trials, se...
متن کاملReclassification of the Genus Chitinotylenchus (Micoletzky, 1922) and a Redescription of C. paragracilis (Micoletzky, 1922) (Nematoda: Tylenchoidea).
The holotype of Chitinotylenchus paragracilis (Micoletzky, 1922) is redescribed and illustrated. Chitinotylenchus is proposed as a synonym of Ditylenchus Filipjev, 1936 and C. paragracilis as Ditylenchus paragracilis (Micoletzky, 1922) new comb. The four other species in the genus Chitinotylenchus are considered species inquirendae.
متن کاملFreezing and Storing Ditylenchus dipsaci in Liquid Nitrogen.
After 18 months of storage at -150 C, some larvae of Ditylenchus dipsaci, which had been treated in a 7.5% solution of dimethyl sulphoxide and cooled to -25 C before storage, were still viable on thawing. Some survivors penetrated and developed normally in stems of alfalfa seedlings. Tests showed that active larvae could be frozen directly, thus eliminating the need to use the quiescent stage o...
متن کاملControl of Ditylenchus dipsaci in Infected Garlic Seed Cloves by Nonfumigant Nematicides.
Different rates of granular formulations ofaldicarb, carbofuran, ethoprop, fensulfothion, and phenamiphos were applied directly onto garlic seed cloves in the seed furrow in sandy clay loam, clay loam, and loam soils at planting to assess efficacy for control of Ditylenchus dipsaci in infected seed cloves. All treatments were compared to hotwater-formalin clove dip disinfection treatment and to...
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1. ACEDO, J. R., and R. A. R O H D E . 1971. His tochemica l root pa thology of Brassica oleracea capi ta ta L. infected by Pra ty lenchus pene t rans (Cobb) Filipjev and S c h u u r m a n s Stekhoven (Nematoda : Tylenchida) . J. Nemato l . 3:62-68. 2. B H A T I A , I. S., J. S INGH, and K. L. BAJAJ. 1973. A new chromogen ic r eagen t for the detect ion of phenol ic c o m p o u n d s on th inla...
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